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- They were the first commercially important producer of restriction enzymes, they were the first supplier of Klenow enzyme for Sanger sequencing, and they are the only supplier of a system for the non-radioactive labeling of nucleic acid.
- All three of these sequencing centers have shifted from Sanger sequencing to next-generation sequencing (NGS).
- Illumina uses next-generation sequencing, which is far faster and more efficient than traditional Sanger sequencing.
- 1 has the ability to perform Sanger Sequencing and Next Generation Sequencing.
- Direct DNA sequencing is currently considered the best method of HLA typing, either by Sanger sequencing or next generation sequencing, though it can also be time-consuming and is one of the more expensive methods.
- Currently, the most accurate test to determine if an individual is affected by lymphedema-distichiasis syndrome is done via Sanger sequencing, which includes whole genome analysis and single gene and multigene testing.
- It used the Sanger sequencing method, a technology which formed the basis of the "first generation" of DNA sequencers while a human genome can be sequenced at 15 times coverage in a matter of days.
- ... g. Sanger sequencing), preferably by using the same primer on the DNA template strand.
- Recent studies use "shotgun" Sanger sequencing or massively parallel pyrosequencing to get largely unbiased samples of all genes from all the members of the sampled communities.
- High-throughput sequencing characterization of RACE fragments is highly time-efficient, more sensitive, less costly and technically feasible compared to traditional characterization of RACE fragments with molecular cloning followed by Sanger sequencing of a few clones.
- ... g. Sanger sequencing). This can make the process of genome assembly more difficult, particularly for sequences containing a large amount of repetitive DNA.
- The chain-termination method of DNA sequencing ("Sanger sequencing") can only be used for short DNA strands of 100 to 1000 base pairs.
- Using this method, up to 17 gigabases can be sequenced at once, as opposed to limited ranges for Sanger sequencing of only about 1 kilobase.
- This new sequencing method generated reads much shorter than those of Sanger sequencing: initially about 100 bases, now 400-500 bases.
- NEMO is a subunit of the IκB kinase complex that activates NF-κB. Sanger sequencing has indicated two distinct variations in the 3’ UTR region of the NEMO gene in preeclamptic women (IKBKG:c.*368C>A and IKBKG:c.*402C>T).
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