Übersetzung für 'T7 RNA polymerase' von Englisch nach Deutsch

T7 RNA polymerase

T7-RNA-Polymerase {f}biol.

• The special properties of the HDV ribozyme's cleavage reaction make it a useful tool to prepare RNA transcripts with homogenous 3′ ends, an alternative to transcription of RNA with T7 RNA polymerase than can often produce heterogenous ends or undesired additions.
• The DE3 prophage found in BL21(DE3) provides T7 RNA polymerase (driven by the LacUV5 promoter), allowing for vectors with the T7 promoter to be used instead.
• Usually, each member of this DNA library has a T7 RNA polymerase transcription site and a ribosomal binding site at the 5’ end.
• Eigen built on Spiegelman's work and produced a similar system further degraded to just 48 or 54 nucleotides—the minimum required for the binding of the replication enzyme, this time a combination of HIV-1 reverse transcriptase and T7 RNA polymerase.
• Termination efficiency of T7 RNA polymerase is around 74%, which creates issues when T7 RNA polymerase is used to produce recombinant proteins.

  ---

• The T7 promoter sequence is used extensively in molecular biology due to its extremely high affinity for T7 RNA polymerase and thus high level of expression.
• The polymerases used in RCA are Phi29, Bst, and Vent exo-DNA polymerase for DNA amplification, and T7 RNA polymerase for RNA amplification.
• Richardson's most highly-cited accomplishment was made while working with bacteriophage T7 RNA polymerase in 1985.
• Modern "lacUV5" is seen in the BL21(DE3) strain, which carries both a "lac" operon with the standard promoter and a "lacUV5" operon split by the DE3 prophage (and as a result driving the T7 RNA polymerase instead).
• The crystal structure of the LC ribozyme was recently published. In brief, a circularly permutated (CP) ribozyme RNA was generated by in vitro transcription using T7 RNA polymerase.

  ---