⇄ 

Übersetzung für 'Puromycin' von Englisch nach Deutsch

puromycin [C22H29N7O5]

Puromycin {n}pharm.

• Puromycin {n} = puromycin [C22H29N7O5]

• PCR DNA is first transcribed to mRNA, and a single-stranded DNA oligonucleotide modified with biotin and puromycin on each end is then hybridized to the 3’-end of the mRNA.
• It is competitive with puromycin suggesting a highly similar binding site.
• Thus, the two substrates of this enzyme are S-adenosyl methionine and O-demethylpuromycin, whereas its two products are S-adenosylhomocysteine and puromycin.
• Through various studies in which functional derivatives of ribosomes were mixed with puromycin (an analog of the 3' end of an aa-tRNA) it was determined that adding LepA to a post transcriptionally modified ribosome prevents dipeptide formation as it inhibits the binding of aa-tRNA to the A site.
• Puromycin is an analogue of the 3’ end of a tyrosyl-tRNA with a part of its structure mimics a molecule of adenosine, and the other part mimics a molecule of tyrosine.

• It contains resistance genes against ampicillin and puromycin.
• This puromycin-sensitive enzyme is Co2+-activated zinc-sialoglycoprotein.
• Puromycin-associated nascent chain proteomics (PUNCH-P) uses a puromycin-biotin label to capture nascent polypeptides for MS.
• One method uses compounds such as harringtonine, puromycin or lactimidomycin to stop ribosomes at translation initiation sites.
• Puromycin is a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase (metallopeptidase).

• "Streptomyces alboniger" produces puromycin, a type of antibiotic.